Anti-Nestin Rabbit pAb, Primary Antibody

Anti-Nestin Rabbit pAb, Primary Antibody

Antibodies Oncology
AO-03-GB115685-50

Anti-Nestin Rabbit pAb

Volume : 50 µL
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299,00 €
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Product Information

Protein full name

Nestin

Synonyms

NES, Nbla00170, nestin

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Nestin

Uniprot ID

P48681

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, intermediate filament cytoskeleton

Applications

IHC/IF

Human

1: 500-1: 1000

glioblastoma multiforme, kidney

Background

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Images

01 GB115685_Nestin-C3359-C335923082202_d500_H glioblastoma multiforme_20.0x_IHC_WM.png

IHC analysis of Nestin(GB115685).
Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃ 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB115685_Nestin-C3359-C335923082202_d500_H kidney_20.0x_IHC_WM.png

IHC analysis of Nestin(GB115685).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃ 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB115685-50
: 10 Produits
Hurry! only 10 items left in stock.

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