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Product Information
|
Description |
IL-6 rabbit polyclonal antibody |
|
Protein full name |
Interleukin-6 |
|
Synonyms |
IL6, BSF2, HGF, HSF, IFNB2, IL-6, BSF-2, CDF, IFN-beta-2, interleukin 6 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse IL-6 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Secreted |
|
Predicted MW. |
24 kDa |
|
Observed MW. |
24 kDa |
|
Uniprot ID |
P08505 |
Applications
|
WB |
Mouse |
1: 500-1: 1000 |
Raw264.7 |
|
IHC/IF |
Mouse, Rat |
1: 200-1: 1600 |
lung, lung+LPS, spleen |
Background
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Images
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WB analysis of IL6 (GB11117). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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WB analysis of IL6 (GB11117). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
 |
IHC analysis of IL-6 (GB11117). Sample: Mouse lung+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IF analysis of IL-6 (GB11117). Sample: Mouse spleen+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
 |
IF analysis of IL-6 (GB11117). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
 |
IF analysis of IL-6 (GB11117). Sample: Rat spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB11117-50
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