Anti-Cyclin E1 Rabbit pAb

Protein full name

G1/S-specific cyclin-E1

Synonyms

CCNE1, CCNE, pcyclin E1, Cyclin E1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclin E1

Uniprot ID

Q61457

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

47 kDa / 47 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 200-1: 400

HeLa, MCF7, C6

IHC/IF

Human, Mouse, Rat

1: 100-1: 400

testis

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis.

Images

WB analysis of Cyclin E1(GB111936).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of Cyclin E1(GB111936).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Cyclin E1(GB111936).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.