Recombinant Anti-CDK1 antibody (Rabbit mAb)

Protein full name

Cyclin-dependent kinase 1

Synonyms

CDK1, CDC2, CDC28A, P34CDC2, Cell division protein kinase 1, Cyclin dependent kinase 1, p34 protein kinase

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CDK1

Uniprot ID

P06493

Isotype

IgG

Clone Number

SB643

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm, Cytoskeleton

Predicted MW./Observed MW.

34 kDa / 34 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

293, A549, MCF7

IHC/IF

Human, Mouse, Rat

1: 2000-1: 4000

esophageal cancer, tonsil, testis

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Cyclin-dependent kinase 1 also known as CDK1 or cell division cycle protein 2 homolog is a highly conserved protein that functions as a serine/threonine kinase, and is a key player in cell cycle regulation.Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins.

Images

WB analysis of CDK1(GB15634).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of CDK1(GB15634).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of CDK1(GB15634)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.