Recombinant Anti-Aggrecan antibody (Rabbit mAb) - 100 µL

Product Information

Protein full name

Aggrecan core protein

Synonyms

ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, Aggrecan

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Aggrecan

Uniprot ID

P16112

Isotype

IgG

Clone Number

SB525

Purity

Affinity purification

Predicted MW./Observed MW.

261 kDa / 250-400 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 1000-1: 2000

SW1353

Storage

Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

background

Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the lectican (chondroitin sulfate proteoglycan) family. This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Images

WB analysis of Aggrecan(GB155753).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.