Recombinant Anti-Sp7/Osterix antibody (Rabbit mAb) - 100 µL

Product Information

Protein full name

Transcription factor Sp7

Synonyms

C22, Zinc finger protein osterix, Sp7, Osx, Osterix, Sp7 transcription factor

Immunogen

Recombinant protein corresponding to Human Sp7

Uniprot ID

Q8TDD2

Isotype

IgG

Clone Number

SB491

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW. / Observed MW.

45 kDa / 45 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 2000

Saos-2

IHC/IF

Human, Mouse, Rat

1: 1500-1: 3000

embryo, bone

Storage

Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

background

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.

Images

WB analysis of Sp7/Osterix(GB151900).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Sp7/Osterix(GB151900).
Sample: Mouse embryo, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100°C 25min.
Primary antibody: 1: 1500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Sp7/Osterix(GB151900)red.
Sample: Rat bone, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100°C 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.