Product Information
Protein full name | Osteopontin |
Synonyms | Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, BSPI, ETA-1, Osteopontin |
Uniprot ID | P10923 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Osteopontin |
Isotype | IgG1 |
Clone Number | 3H1D12 |
Predicted MW./Observed MW. | 32 kDa / 44-75 kDa |
Purity | Affinity purification |
Subcellular location | Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse | 1: 500-1: 1000 | A549, RAW264.7, kidney |
| IHC/IF | Mouse, Rat | 1: 300-1: 600 | bone |
Background
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by theSPP1 gene (secreted phosphoprotein 1).Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite.
Images
| WB analysis of Osteopontin(GB120018). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rat IgG (H+L) (GB23302),1: 5000, RT, 1h. |
| IHC analysis of Osteopontin(GB120018). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rat IgG (H+L) (GB23302),1: 200, RT, 1h. |
| IHC analysis of Osteopontin(GB120018). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rat IgG (H+L) (GB23302),1: 200, RT, 1h. |
| IHC analysis of Osteopontin(GB120018). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rat IgG (H+L) (GB23302),1: 200, RT, 1h. |
| IF analysis of Osteopontin(GB120018)red. Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rat IgG (H+L) (GB21302), 1: 300, RT, 1h. |
| IF analysis of Osteopontin(GB120018)red. Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rat IgG (H+L) (GB21302), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Datasheet: Download datasheet 1
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