Anti-Osteopontin Rabbit pAb - 50 µL

Product Information

Protein full name

Osteopontin

Synonyms

Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, BSPI, ETA-1, Osteopontin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Osteopontin

Uniprot ID

P10923

Isotype

IgG

Purity

Affinity purification

Predicted MW./Observed MW.

32 kDa / 44-75 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, kidney

Storage

Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

background

Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by theSPP1 gene (secreted phosphoprotein 1).Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite.

Images

WB analysis of Osteopontin(GB112328).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.