EasyStep Human APOB100(Apolipoprotein B100) ELISA Kit
AO-06-ELK102ES-48T
| Cat.No | ELK102ES |
| Product Name | EasyStep Human APOB100(Apolipoprotein B100) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Apo-B100 |
| Assay Type | Sandwich |
| Sensitivity | 41 ng/mL |
| Standard | 10 μg/mL |
| Detection Range | 0.16-10 μg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB100, and the Human APOB100 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human APOB100. After TMB substrate solution is added, only those wells that contain Human APOB100 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve. |

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