Human HSF2(Heat Shock Transcription Factor 2) ELISA Kit

Human HSF2(Heat Shock Transcription Factor 2) ELISA Kit

ELK Biotechnology
AO-06-ELK2964-48T

Human HSF2(Heat Shock Transcription Factor 2) ELISA Kit 48T

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Product Overview

Cat.No ELK2964
Product Name Human HSF2(Heat Shock Transcription Factor 2) ELISA Kit
Reactivity Human
Alternative Names HSTF2; Heat shock factor protein 2
Assay Type Sandwich
Sensitivity 0.054 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Assay Length 3.5h
Research Area Signal transduction;Developmental science;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK2964-b-1.jpg
: AO-06-ELK2964-48T
: 10 Produits
Hurry! only 10 items left in stock.

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