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Human hs-Hepc25(high sensitivity Hepcidin 25) Microsample ELISA Kit
AO-06-ELK9112MS-96T
Product Overview
| Cat.No | ELK9112MS |
| Product Name | Human hs-Hepc25(high sensitivity Hepcidin 25) Microsample ELISA Kit |
| Reactivity | Human |
| Alternative Names | Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor; |
| Assay Type | Sandwich |
| Sensitivity | 0.61 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cardiovascular; Signal Transduction; Cardiovascular; Metabolism |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9112MS-96T
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