Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) Microsample ELISA Kit

Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) Microsample ELISA Kit

AO-06-ELK1264MS-48T

Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) Microsample ELISA Kit 48T

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Product Overview

Cat.No ELK1264MS
Product Name Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) Microsample ELISA Kit
Reactivity Human
Alternative Names FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type Sandwich
Sensitivity 0.06 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK1264MS-b-1.jpg
: AO-06-ELK1264MS-48T
: 10 Produits
Hurry! only 10 items left in stock.

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