Human INHbB(Inhibin Beta B) Microsample ELISA Kit

Human INHbB(Inhibin Beta B) Microsample ELISA Kit

AO-06-ELK2175MS-48T

Human INHbB(Inhibin Beta B) Microsample ELISA Kit 48T

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Product Overview

Cat.No ELK2175MS
Product Name Human INHbB(Inhibin Beta B) Microsample ELISA Kit
Reactivity Human
Alternative Names INH-BB; Activin AB Beta Polypeptide
Assay Type Sandwich
Sensitivity 5.4 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Type Serum, plasma, tissue homogenates and other biological fluids.
Assay Length 3.5h
Research Area Tumor immunity;Endocrinology;Reproductive science;Developmental science;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human INHbB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human INHbB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human INHbB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human INHbB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK2175MS-b-1.jpg
: AO-06-ELK2175MS-48T
: 10 Produits
Hurry! only 10 items left in stock.

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