Human LIN28A(Lin-28 Homolog A) ELISA Kit

Human LIN28A(Lin-28 Homolog A) ELISA Kit

ELK Biotechnology
AO-06-ELK7642-48T

Human LIN28A(Lin-28 Homolog A) ELISA Kit 48T

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Product Overview

Cat.No ELK7642
Product Name Human LIN28A(Lin-28 Homolog A) ELISA Kit
Reactivity Human
Alternative Names ZCCHC1; CSDD1; LIN28; Zinc finger CCHC domain-containing protein 1
Assay Type Sandwich
Sensitivity 22.2 pg/mL
Standard 4000 pg/mL
Detection Range 62.5-4000 pg/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Assay Length 3.5h
Research Area Signal transduction;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIN28A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIN28A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LIN28A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIN28A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

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: AO-06-ELK7642-48T
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Hurry! only 10 items left in stock.

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