Human LONP1(Lon protease homolog, mitochondrial) ELISA Kit

Human LONP1(Lon protease homolog, mitochondrial) ELISA Kit

AO-06-ELK9460-48T

Human LONP1(Lon protease homolog, mitochondrial) ELISA Kit 48T

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Product Overview

Cat.No ELK9460
Product Name Human LONP1(Lon protease homolog, mitochondrial) ELISA Kit
Reactivity Human
Alternative Names Lon protease homolog, mitochondrial; LONHs; Lon protease-like protein; LONP; Mitochondrial ATP-dependent protease Lon; Serine protease 15; PRSS15; LONP1
Assay Type Sandwich
Sensitivity 12.2 pg/mL
Standard 2000 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Epigenetics and Nuclear Signaling; Metabolism; Cancer
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LONP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LONP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LONP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LONP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK9460-b-1.jpg
: AO-06-ELK9460-48T
: 10 Produits
Hurry! only 10 items left in stock.

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