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Human MEP1a(Meprin A Alpha) ELISA Kit
AO-06-ELK5367-48T
Product Overview
| Cat.No | ELK5367 |
| Product Name | Human MEP1a(Meprin A Alpha) ELISA Kit |
| Reactivity | Human |
| Alternative Names | PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase |
| Assay Type | Competitive Inhibition |
| Sensitivity | 17 pg/mL |
| Standard | 4000 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK5367-48T
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