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Human MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit
AO-06-ELK3010-96T
Product Overview
| Cat.No | ELK3010 |
| Product Name | Human MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit |
| Reactivity | Human |
| Alternative Names | HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8 |
| Assay Type | Sandwich |
| Sensitivity | 13.3 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | serum, plasma, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK3010-96T
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