Human MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit

Human MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit

AO-06-ELK1117MS-96T

Human MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit 96T

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Product Overview

Cat.No ELK1117MS
Product Name Human MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit
Reactivity Human
Alternative Names MIP1 Beta; CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b; CCL4L1; C C motif chemokine ligand 4 like 1; CC chemokine ligand 4L1; C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; CCL4L; SCYA4L1; CCL4L2; SCYA4L2
Assay Type Sandwich
Sensitivity 6.2 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Cytokine;Infection immunity;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK1117MS-b-1.jpg
: AO-06-ELK1117MS-96T
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Hurry! only 10 items left in stock.

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