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Human PADI2(Peptidyl Arginine Deiminase Type II) ELISA Kit
AO-06-ELK4782-48T
Product Overview
| Cat.No | ELK4782 |
| Product Name | Human PADI2(Peptidyl Arginine Deiminase Type II) ELISA Kit |
| Reactivity | Human |
| Alternative Names | PAD-H19; PAD2; PDI2; Peptidylarginine deiminase II |
| Assay Type | Sandwich |
| Sensitivity | 0.66 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PADI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PADI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PADI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PADI2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK4782-48T
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