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Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
AO-06-ELK11308-48T
Product Overview
| Cat.No | ELK11308 |
| Product Name | Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit |
| Reactivity | Human |
| Alternative Names | p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1 |
| Assay Type | Sandwich |
| Sensitivity | 0.121 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11308-48T
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