| Cat.No | ELK8570MS |
| Product Name | Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) Microsample ELISA Kit |
| Reactivity | Human |
| Alternative Names | PIVKA-II; PIVKA II |
| Assay Type | Sandwich |
| Sensitivity | 0.13 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Hematology |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve. |
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English