| Cat.No | ELK9134MS |
| Product Name | Human sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) Microsample ELISA Kit |
| Reactivity | Human |
| Alternative Names | CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1 |
| Assay Type | Sandwich |
| Sensitivity | 12.4 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.3-2000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Immunology |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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