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Human WWOX(WW domain-containing oxidoreductase) ELISA Kit
AO-06-ELK9495-96T
Product Overview
| Cat.No | ELK9495 |
| Product Name | Human WWOX(WW domain-containing oxidoreductase) ELISA Kit |
| Reactivity | Human |
| Alternative Names | WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase |
| Assay Type | Sandwich |
| Sensitivity | 0.113 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cancer |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WWOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WWOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WWOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WWOX in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9495-96T
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