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Human y+LAT1(L-Type Amino Acid Transporter 1) ELISA Kit
AO-06-ELK4710-96T
Product Overview
| Cat.No | ELK4710 |
| Product Name | Human y+LAT1(L-Type Amino Acid Transporter 1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | SLC7A7; LPI; Y+LAT1; MOP-2; Monocyte amino acid permease 2; Y+L Amino Acid Transporter 1; Solute Carrier Family 7 Member 7,Cationic Amino Acid Transporter,y+ System |
| Assay Type | Sandwich |
| Sensitivity | 0.118 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Signal transduction; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human y+LAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human y+LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human y+LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human y+LAT1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK4710-96T
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