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Human 5-MTHF(5-Methyltetrahydrofolate) ELISA Kit
AO-06-ELK0833-96T
Product Overview
| Cat.No | ELK0833 |
| Product Name | Human 5-MTHF(5-Methyltetrahydrofolate) ELISA Kit |
| Reactivity | Human |
| Alternative Names | L-5-MTHF; 5-MTHF; |
| Assay Type | Sandwich |
| Sensitivity | 0.059 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Reproductive science;Genetic science;Nutrition metabolism; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5-MTHF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-MTHF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5-MTHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-MTHF in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0833-96T
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