| Cat.No | ELK11340 |
| Product Name | Human Anti-CD74(Anti-HLA class II histocompatibility antigen gamma chain Antibody) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CD74 antibody;DHLAG antibody |
| Assay Type | Sandwich |
| Sensitivity | 0.63 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Immunology |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-CD74. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-CD74. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-CD74, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-CD74 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English