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Human E-Cad(E-Cadherin) ELISA Kit
AO-06-ELK11056-96T
Product Overview
| Cat.No | ELK11056 |
| Product Name | Human E-Cad(E-Cadherin) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial |
| Assay Type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma, urine, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | CD & Adhesion molecule;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11056-96T
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