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Product Overview
| Cat.No | ELK0737 |
| Product Name | Human ELA(Elabela) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Protein Elabela; Protein Toddler; Apelin receptor early endogenous ligand |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.61 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Cardiovascular biology; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ELA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0737-96T
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