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Mouse 4-HNE(4-Hydroxynonenal) Microsample ELISA Kit
AO-06-ELK8374MS-48T
Product Overview
| Cat.No | ELK8374MS |
| Product Name | Mouse 4-HNE(4-Hydroxynonenal) Microsample ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal |
| Assay Type | Sandwich |
| Sensitivity | 1.1 ng/mL |
| Standard | 150 ng/mL |
| Detection Range | 2.35-150 ng/mL |
| Sample Type | Serum, plasma and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK8374MS-48T
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