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Mouse 5-HT(5-Hydroxytryptamine) ELISA Kit
AO-06-ELK9288-48T
Product Overview
| Cat.No | ELK9288 |
| Product Name | Mouse 5-HT(5-Hydroxytryptamine) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | 5HT; Serotonin; N-Methyl-Gamma |
| Assay Type | Competitive Inhibition |
| Sensitivity | 3.93 ng/mL |
| Standard | 900 ng/mL |
| Detection Range | 14.07-900 ng/mL |
| Sample Type | serum, plasma |
| Assay Length | 2h |
| Research Area | Signal transduction;Infection immunity;Neuro science;Gastroenterology; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK9288-48T
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