Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit

Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit

AO-06-ELK4340-96T

Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit 96T

check En Stock
Hurry! only 10 items left in stock.
489,00 €
HT
Quantité

Product Overview

Cat.No ELK4340
Product Name Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Reactivity Mouse
Alternative Names NFKBIA; IKB-A; MAD-3; NFKBI; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Alpha; Major histocompatibility complex enhancer-binding protein MAD3
Assay Type Sandwich
Sensitivity 0.057 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Tissue homogenates, cell lysates and other biological fluids.
Assay Length 3.5h
Research Area Signal transduction;Tumor immunity;Hematology;Developmental science;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK4340-b-1.jpg
: AO-06-ELK4340-96T
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package