Mouse IL-27(Interleukin 27) ELISA Kit

Mouse IL-27(Interleukin 27) ELISA Kit

AO-06-ELK9612-96T

Mouse IL-27(Interleukin 27) ELISA Kit 96T

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Product Overview

Cat.No ELK9612
Product Name Mouse IL-27(Interleukin 27) ELISA Kit
Reactivity Mouse
Alternative Names IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;
Assay Type Sandwich
Sensitivity 18.27 pg/mL
Standard 2000 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length 3.5h
Research Area Immunology;Cancer
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK9612-b-1.jpg
: AO-06-ELK9612-96T
: 10 Produits
Hurry! only 10 items left in stock.

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