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Mouse ITGb1(Integrin Beta 1) Microsample ELISA Kit
AO-06-ELK5301MS-48T
Product Overview
| Cat.No | ELK5301MS |
| Product Name | Mouse ITGb1(Integrin Beta 1) Microsample ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta |
| Assay Type | Sandwich |
| Sensitivity | 0.113 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | CD & Adhesion molecule;Tumor immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGb1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK5301MS-48T
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