Mouse NES1(Nesfatin 1) ELISA Kit

Mouse NES1(Nesfatin 1) ELISA Kit

AO-06-ELK5437-48T

Mouse NES1(Nesfatin 1) ELISA Kit 48T

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Product Overview

Cat.No ELK5437
Product Name Mouse NES1(Nesfatin 1) ELISA Kit
Reactivity Mouse
Alternative Names Nesfatin-1
Assay Type Competitive Inhibition
Sensitivity 193.2 pg/mL
Standard 40000 pg/mL
Detection Range 625-40000 pg/mL
Sample Type serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length 2h
Research Area Glycolipid metabolism
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

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: AO-06-ELK5437-48T
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Hurry! only 10 items left in stock.

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