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Mouse PRNP(Prion Protein) ELISA Kit
AO-06-ELK5103-48T
Product Overview
| Cat.No | ELK5103 |
| Product Name | Mouse PRNP(Prion Protein) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CD230; GSS; ASCR; CJD; PRIP; PrP; PrP2730; PrPc; Major prion protein; Creutzfeldt-Jakob Disease; Gerstmann-Strausler-Scheinker Syndrome; Fatal Familial Insomnia |
| Assay Type | Sandwich |
| Sensitivity | 0.058 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction;CD & Adhesion molecule;Infection immunity;Neuro science; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRNP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK5103-48T
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