Mouse S100A11(S100 Calcium Binding Protein A11) ELISA Kit

Mouse S100A11(S100 Calcium Binding Protein A11) ELISA Kit

AO-06-ELK10462-48T

Mouse S100A11(S100 Calcium Binding Protein A11) ELISA Kit 48T

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Product Overview

Cat.No ELK10462
Product Name Mouse S100A11(S100 Calcium Binding Protein A11) ELISA Kit
Reactivity Mouse
Alternative Names S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed
Assay Type Sandwich
Sensitivity 0.059 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length 3.5h
Research Area Tumor immunity;Infection immunity;Developmental science;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A11 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

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: AO-06-ELK10462-48T
: 10 Produits
Hurry! only 10 items left in stock.

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