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Mouse sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
AO-06-ELK11157-48T
Product Overview
| Cat.No | ELK11157 |
| Product Name | Mouse sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9 |
| Assay Type | Sandwich |
| Sensitivity | 13.22 ng/mL |
| Standard | 2000 ng/mL |
| Detection Range | 31.25-2000 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK11157-48T
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