Mouse YY1(YY1 Transcription Factor) ELISA Kit

Mouse YY1(YY1 Transcription Factor) ELISA Kit

AO-06-ELK0981-48T

Mouse YY1(YY1 Transcription Factor) ELISA Kit 48T

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Product Overview

Cat.No ELK0981
Product Name Mouse YY1(YY1 Transcription Factor) ELISA Kit
Reactivity Mouse
Alternative Names NF-E1; DELTA; UCRBP; YIN-YANG-1; INO80S; INO80 Complex Subunit S; Yin and Yang 1 Protein; Delta transcription factor; INO80 complex subunit S
Assay Type Sandwich
Sensitivity 0.059 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Assay Length 3.5h
Research Area Immune molecule;
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse YY1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse YY1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse YY1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse YY1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

ELK0981-b-1.jpg
: AO-06-ELK0981-48T
: 10 Produits
Hurry! only 10 items left in stock.

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