Human Interleukin-33, IL33 ELISA Kit

Human Interleukin-33, IL33 ELISA Kit

Primacu
AO-06-BPE073

Size: 96T

Sensitivity: 0.29 pg/mL

Detection range: 1.56-100 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

check En Stock
Hurry! only 10 items left in stock.
469,00 €
HT
Quantité

ajouter comparer
Ajouter souhaits

Full product name

Interleukin-33

Code

BPE073

Assay type

Sandwich

Size

15*96T,5*96T,96T

Sensitivity

0.29 pg/mL

Detection range

1.56-100 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

UniProt accession

O95760

MASS（Da）

30759

GeneID

90865

Synonyms

C9orf26;IL1F11;NFHEV

Gene names

IL33

Research area

Cardiovascular,Immunology

Target protein

IL-33

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE073 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
100 pg/ml 2.339 2.308 2.199
2.278
50 pg/ml 1.342 1.365 1.256
1.388
25 pg/ml 0.744 0.768 0.659
0.792
12.5 pg/ml 0.452 0.439 0.33
0.426
6.25 pg/ml 0.253 0.27 0.161
0.288
3.125 pg/ml 0.174 0.183 0.074
0.193
1.5625 pg/ml 0.156 0.15 0.041
0.145
0 pg/ml 0.106 0.109 0
0.113

Precision

We measured random samples of BPE073 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 6.5 0.18 2.8
Intra-assay 2 20 32.6 1.3 3.9
Intra-assay 3 20 65.2 3.3 5
Inter-assay 1 20 6.4 0.46 7.2
Inter-assay 2 20 32.4 2 6.1
Inter-assay 3 20 65.5 4.7 7.2
: AO-06-BPE073
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package