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EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
AO-06-ELK051ES-96T
Product Overview
| Cat.No | ELK051ES |
| Product Name | EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 25 Hydroxy Vitamin D; |
| Assay Type | Competitive Inhibition |
| Sensitivity | 7.7 ng/mL |
| Standard | 5000 ng/mL |
| Detection Range | 78.13-5000 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK051ES-96T
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