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Antibody Product Overview
| ELK.No | EM1066 |
| Product name | NSE Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Other name | ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2026 |
| Human Swiss-Prot | P09104 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:2,000 IHC 1:200 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 47kDa |
| Background | Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. |
| Function | |
| Subcellular location | Cytoplasm. Cell membrane. |
| Expression |
Additional Images
Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates with NSE mAb diluted at 1:3,000.
IHC staining of Human small cell carcinoma of lung tissue with NSEmouse mAb(13E2) diluted at 1:200.
: EM1066-100
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