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Antibody Product Overview
| ELK.No | EM1073 |
| Product name | FH/Fumarase Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IF |
| Other name | Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2271 |
| Human Swiss-Prot | P07954 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:3,000 IF 1:100-200 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 50kDa |
| Background | Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. |
| Function | |
| Subcellular location | Cytoplasm and Mitochondrion. |
| Expression |
Additional Images
Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, with FH Mouse mAb diluted at 1:3,000.
Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, with FH Mouse mAb diluted at 1:3,000.
IF analysis of Hela with EM1073 diluted at 1:100.
: EM1073-100
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