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Antibody Product Overview
| ELK.No | EM1077 |
| Product name | Caspase 9 Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IP, IF |
| Other name | APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 842 |
| Human Swiss-Prot | P55211 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic Peptide |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:1,000-5,000 IP 1:200 IF 1:100-200 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 47（51）kDa |
| Background | Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
| Function | |
| Subcellular location | Cytosol,Mitochondrion,Nucleus |
| Expression |
Additional Images
Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1）1:2,000 2）1:5,000
Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100.
: EM1077-50
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