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Antibody Product Overview
| ELK.No | EM1095 |
| Product name | Cytokeratin 18 Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Other name | Cell proliferation inducing gene 46 protein, CK 18, CYK 18, K 18, Keratin 18, Ketatin D, KRT18 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3875 |
| Human Swiss-Prot | P05783 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Recombinant Protein |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 46kDa |
| Background | Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. |
| Function | |
| Subcellular location | Cytoplasm, Intermediate filament, Keratin, Nucleus |
| Expression |
Additional Images
Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue with Cytokeratin 18 Mouse MAb (1B3) diluted at 1:2000.
IF analysis of Hela with EM1095(Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Cytokeratin 18 （EM1095）Mouse mAb diluted at 1:500.
: EM1095-50
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