Français
English
Antibody Product Overview
| ELK.No | EM1111 |
| Product name | NFkB p65 Mouse mAb |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, IP |
| Other name | NFKB3, p65, RELA, Transcription factor p65 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5970 |
| Human Swiss-Prot | Q04206 |
| Source | Mouse |
| Isotype | IgG1 |
| Target | |
| Fields | |
| Gene name | |
| Protein name | |
| Human gene link | |
| Human Swiss link | |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Recombinant Protein |
| Specificity | |
| Formulation | |
| Clonality | Monoclonal |
| Dilution | WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Purification | |
| Concentration | 1mg/mL |
| Storage stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kDa |
| Background | NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. |
| Function | |
| Subcellular location | Cytoplasm, Nucleus |
| Expression |
Additional Images
Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFκB p65 diluted at 1:1,500.
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1111 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
IF analysis of Hela with EM1111(Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 （EM1111）Mouse mAbdiluted at 1:500.
: EM1111-100
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package