Anti-PCNA Mouse mAb
   

Anti-PCNA Mouse mAb

Antibodies Oncology
AO-03-GB12010-100
12010,AO-03-GB12010,AO-03-GB12010100,ATLD2,PCNA,proliferating cell nuclear antigen,Proliferating cell nuclear antigen
check En Stock
Hurry! only 10 items left in stock.
399,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

Recombinant protein corresponding to Mouse PCNA

Isotype

IgG2a, κ

Clone Number

4C9A9

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 testis, placenta
IHC/IF Human, Mouse, Rat 1: 1000-1: 4000 colon, rectum, spleen
ICC/IF Human, Mouse, Rat 1: 100-1: 500 HeLa



Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01JaFr3gHFIrT8s.jpg

 WB analysis of PCNA(AO-03-GB12010).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 5000, RT, 1h.

025VPo0OEFtdYJK.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Human gastric cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

03pFACFvYiFXWy0.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Human rectum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

0455F5aKeNESBvg.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

05rkdpqMphM7mqn.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

06lYGmNSH22tr8W.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

07lCI4HnZbAlMC4.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

08i9jUYGqCA6VQS.jpg

 IHC analysis of PCNA(AO-03-GB12010).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (AO-03-GB23301), 1: 200, RT, 1h.

09OUmWLWVx5j2bO.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

10NajbOEeAFtLkN.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

11Yqp8BlZHWSlGS.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

12kRtvnFgPfnNF8.jpg

 IF analysis of PCNA(AO-03-GB12010)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

13vq4FM7XZ3Drk5.jpg

 ICC analysis of PCNA(AO-03-GB12010)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.

147AcAlensQRU3y.jpg

 ICC analysis of PCNA(AO-03-GB12010)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (AO-03-GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12010-100
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package