Anti-PCNA Rabbit pAb
   

Anti-PCNA Rabbit pAb

Antibodies Oncology
AO-03-GB11010-100
11010,ATLD2,PCNA,proliferating cell nuclear antigen,AO-03-GB11010,AO-03-GB11010100,Proliferating cell nuclear antigen
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399,00 €
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Ajouter souhaits

Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Isotype

IgG

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 HeLa, SW480, B16
IHC/IF Human, Mouse, Rat 1: 200-1: 1000 intestinal cancer, liver cancer, lung cancer
ICC/IF Human, Mouse, Rat 1: 200-1: 400 HeLa
IP Human, Mouse, Rat 1: 50-1: 200 HeLa cells



Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01zSd5sQNoEGxKq.jpg

 WB analysis of PCNA(AO-03-GB11010).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 5000, RT, 1h.

02CobhH9KsMC3BV.jpg

 WB analysis of PCNA(AO-03-GB11010).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 5000, RT, 1h.

03Dpabi7CIMlswK.jpg

 Immunoprecipitation of HeLa cells lysate using PCNA（IP: AO-03-GB11010, 1: 100; Detection: AO-03-GB12010, 1: 2000）

04LSdLi8yok5jHh.jpg

 IHC analysis of PCNA(AO-03-GB11010).
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

05axrwWXYpe31q2.jpg

 IHC analysis of PCNA(AO-03-GB11010).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (AO-03-GB23303), 1: 200, RT, 1h.

06O4vyVJSFLA10T.jpg

 IF analysis ofPCNA(AO-03-GB11010)red.
Sample: Human intestinal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

07tu26peZ5KPMzK.jpg

 IF analysis ofPCNA(AO-03-GB11010)red.
Sample: Human intestinal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

08InKQvomK0mCxK.jpg

 IF analysis ofPCNA(AO-03-GB11010)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

09ULn66ylAFgKBU.jpg

 IF analysis ofPCNA(AO-03-GB11010)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

10AYphXxmxGrlh2.jpg

 IF analysis ofPCNA(AO-03-GB11010)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.

11iRWX3K8DRelzo.jpg

 ICC analysis of PCNA(AO-03-GB11010)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (AO-03-GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11010-100
: 10 Produits
Hurry! only 10 items left in stock.

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