Anti-NF-κB p65 Rabbit pAb - 50 µL

Product Information

Protein full name

Transcription factor p65

Synonyms

RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit

Immunogen

Recombinant protein corresponding to Mouse Nuclear factor NF-kappa-B p65 subunit

Uniprot ID

Q04207

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Predicted MW. / Observed MW.

65 kDa / 65 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

lung, thymus

ICC/IF

Human, Mouse, Rat

1: 1500-1: 3000

HeLa, NIH/3T3, PC-12

Storage

Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.

Images

WB analysis of NF-κB p65(GB11997).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.