Anti-NF-κB p65 Mouse mAb - 50 µL

Anti-NF-κB p65 Mouse mAb - 50 µL

Antibodies-Oncology
AO-03-GB12142-50
  • Specification: 50 µL
  • Applications: WB
  • Species: H,M,R
  • Dilution: 1: 500-1: 1000
  • Positive tissue: HeLa, HepG2, A549
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Product Information

Protein full name

Transcription factor p65

Synonyms

RELA, NFKB3, p65, RELA proto-oncogene,NF-kB subunit

Uniprot ID

Q04206

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Nuclear factor NF-kappa-B p65 subunit

Isotype

IgG2a, κ

Clone Number

2A7A3

Predicted MW./Observed MW.

65 kDa / 65 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000HeLa, HepG2, A549

Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.

Images

017FlElfvd9v3vI.jpg

 WB analysis of NF-κB p65(GB12142).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02ZhDTs8o2HfnwQ.jpg

 WB analysis of NF-κB p65(GB12142).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB12142-50
: 10 Produits
Hurry! only 10 items left in stock.

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