Anti-TRAF4 Rabbit pAb - 50 µL
   

Anti-TRAF4 Rabbit pAb - 50 µL

Antibodies-Oncology
AO-03-GB111015-50
  • Specification: 50 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | M,R
  • Dilution: 1: 500-1: 1000 | 1: 150-1: 500
  • Positive tissue: thymus, brain, lymph node | thymus, lymphatic, lung
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299,00 €
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Ajouter souhaits

Product Information

Protein full name

TNF receptor-associated factor 4

Synonyms

Cysteine-rich motif associated to RING and Traf domains protein 1, Traf4, Cart1, MLN62, tumor necrosis receptor-associated factor 4A

Uniprot ID

Q9BUZ4

Immunogen

KLH conjugated Synthetic peptide corresponding to Human TRAF4

Isotype

IgG

Predicted MW./Observed MW.

53 kDa / 53 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000thymus, brain, lymph node
IHC/IFMouse, Rat1: 150-1: 500thymus, lymphatic, lung

Background

The tumor necrosis factor receptor-associated factor (TRAF) family is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor super family. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas.

Images

01vOwLnGOcnduOY.jpg

 WB analysis of TRAF4(GB111015).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02YOczWnH5yovjx.jpg

 WB analysis of TRAF4(GB111015).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03JCZ62JLBP3VpL.jpg

 IHC analysis of TRAF4(GB111015).
Sample: Rat thymus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 250, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

040ppGCxuoT9psG.jpg

 IHC analysis of TRAF4(GB111015).
Sample: Rat lymphatic, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 250, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05dfinW18pDSHgE.jpg

 IHC analysis of TRAF4(GB111015).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 250, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

066PGKN4BpyDSiX.jpg

 IHC analysis of TRAF4(GB111015).
Sample: Mouse lymphatic, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 250, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB111015-50
: 10 Produits
Hurry! only 10 items left in stock.

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